Isolation of Casein from Milk and Powdered Milk

Isolation of Casein from Milk and Powdered Milk 1.1 Introduction (Walsh, 2002) stated that, proteins are biological macromolecules composed of amino acids proteins consist of one or more polypeptide which are the chain of amino acids interconnected by peptide bonds.Alberts et al., (2013) detailed that, amino acids of proteins is either hydrophobic or hydrophilic in nature. Therefore the resulting polypeptide chain shows an amphipathic characteristic. Hydrophilic amino acids exist peripherally in some biological system and they are highly water soluble. Whereas some amino acid does not exist the polar groups to the environment. ‘’The most important factors that influence protein solubility are structure, size, charge and the solvent‘’ (Burgess, and Deutscher, 2009). Also Burgess, and Deutscher (2009) stated that, once the precipitation obtained, the solution can be separated by centrifugation or precipitation. ‘’Protein precipitates are aggregates of protein molecular large enough to be visible and to be collected by centrifugation. The distribution of hydrophilic and hydrophobic residues at the surface of a protein determines its solubility properties‘’. (Rosenburg, 2006). Precipitation is mainly done for concentrate the target protein. And it is attained by adding reagents such as salts (ammonium sulfate) or organic solvents (acetone or ethanol). (Hatti-Kaul and Mattiasson, 2003) 1.1.1 Isolation of casein Milk contains three kinds of proteins: caseins, lactalbumins, and lactoglobulins, all of which are globular proteins. (Spurlock, 2014). Ahluwalia and Dhingra, (2005) stated that, Casein is a combination of phosphoproteins presenting in milk and cheese.it is existing to the amount of 3% in milk along with 4-5% of lactose and 3-4% of fats and the rest is water. Caseins exist in micelles which are composed of sub micelles linked by the characteristic of hydrocolloid which are freely suspended in the aqueous phase of milk. (Tarte, 2009). Casein can be electrophoretically fractioned into four major components: alpha-, beta-, gamma-and kappa- casein. Casein develops precipitation from milk at pH 4.6, which has a negative charge when compare to the pH of the milk. Therefor it can be precipitate as salt by adding acids. (Miller, Jarvis and McBean, 2006). 1.2 Objectives To learn the methods of protein precipitation and to relate the solubility of protein with its structure. To learn the methods of isolation of casein from milk and to determine the percentage of casein presented in the (powdered) milk. 1.3 Materials Test tubes Beakers Pipette Clamp Filtering paper Electronic balance Watch glass Bunsen burner Albumin sample Ammonium sulfate Sodium hydroxide Copper sulfate Ethanol Picric acid Lead nitrate Powdered milk Warm water 1.4 Methodology 1.4.1 Precipitation by salts Albumin, 3.00ml was taken into a test tube, ammonium sulfate was added to it and was mixed until the solution gets saturated. The solution was allowed to stand for about 5 minutes and filtered by using filter paper. The biuret test was done to the filtered solution. 3.00 ml of filtered solution was taken into another test tube and same amount of NaOH was added to it, CuSO4 was added drop by drop. 1.4.2 Precipitation by organic solvents Albumin, 1.00 ml was taken into a test tube using a pipette. And 4.00 ml of ethanol was added .the solution was mixed well and was allowed to stand. 1.4.3 Precipitation by acidic agents Picric acid solution, 1.00 ml was added into 1.00ml of albumin solution. 1.4.4 Precipitation by heavy metal ions Lead nitrate, 8 drops were added into 1.00 ml of albumin. 1.4.5 Precipitation by heat and acid Albumin, 10 ml was taken into a test tube and the upper part of the solution was held over the Bunsen flame. After the observation few drops of 1% acetic acid were added. 1.4.6 Isolation of casein Powdered milk (non-fat), 17.5 g was weighed by using electronic balance and was dissolved by adding 62.5 ml of warm water in a 200ml beaker. Acetic acid (10%) was added in a drop wise manner with stirring until the liquid changes in to clear solution. the obtained solution was filtered by using clamp, filtering material and beaker. The yield casein was allowed to dry and was weighed using electronic balance. Biuret test was done for the filtered solution. 3.00 ml of filtered solution was taken into another test tube and same amount of NaOH was added to it, CuSO4 was added drop by drop. 1.5 Results Test Observation Interference Precipitation by metal ions White color precipitation Proteins can be precipitated by metal ions(positive for proteins) Precipitation by heat and acid Initially cloudy white precipitation was observed on the upper part of the solution and by adding acetic acid white color precipitation was observed. Proteins can be precipitate by heat and acid(positive for proteins) Precipitation by organic solvents White color precipitation was observed Proteins can be precipitate by organic solvents(positive for proteins) Precipitation by acidic agent White color precipitation was observed Proteins can be precipitate by acidic agents. (positive for proteins) Precipitation of salts Biuret test White color precipitation was observed. Purple color ring was observed Proteins can be precipitate by salts. Positive for proteins. Isolation of casein Biuret test for filtration Casein 13.01g was weighed Purple color ring was formed in filtered casein solution Yield %= Ãâ€" 100 = Ãâ€" 100 = 74.30% Positive for proteins. 1.6 Discussions Precipitation of protein can be obtained by isoelectric precipitation method. ‘’isoelectric precipitation is the most widely used method’ (Fox and McSweeney, 2003). Proteins can be precipitated by bringing their pH to its isoelectric point in which protein solubility is very low. (Shankara, 2008) Proteins can be precipitate by salts in two ways, half saturation with ammonium sulfate and full saturation with ammonium sulfate. Rashmi, (2002) stated that, different proteins show different precipitation reaction towards diverse agents. The full saturation with ammonium sulfate was done in the laboratory. Also the filtrate was tested by biuret reagent, resulted purple color. ‘Compounds with two or more peptide bonds give a violet color with alkaline copper sulfate’ (Rashmi, 2002) Proteins are strong in solution when they are enclosed by entirely hydrogen-bonded water molecules, as water molecules with additional hydrogen bonding ability have greater entropy and are more aggressive. (Chaplin, 2014) hydrated sphere decrease the non -polarity. Higher the diameter of the sphere higher the solubility. For an example, it is easy to precipitate globulin from proteins by adding salts, than albumin because globulin has small diameter of hydrated sphere when compare to albumin. The similar concept is used in precipitating proteins by organic solvents and acidic agents. Organic solvents remove the hydrated sphere and decrease solubility resulting increase precipitation. Acids neutralize the polarity of the hydrated sphere and decrease solubility in order to increase precipitation. Denaturation occur on heating or adding acidic agents to proteins. Therefor its change the polarity of a protein by changing the arrangements of polar and non-polar groups within the molecule. Less polarity decrease the solubility and increases the precipitation. Precipitation by heavy metal ions lead nitrate was used instead of lead acetate or mercuric nitrate. Shankara(2008) stated that, metal ions which are positively charged interrelate with negatively charged groups of the protein producing precipitation as metal-proteinate complex. According to the percentage of yield and from the result of biuret test of the filtrate, there can be some proteins present in the filtrate. Because, the milk contains about 3.5% protein by weight and of the total protein, about 80% is casein and 20% is whey protein. (Miller, Jarvis and McBean, 2006) Filtration of casein can be done in two ways. Such as, gravitational filtration and sucktional filtration. 1.7. Conclusion Proteins were precipitated by using metal ions, heat, organic solvents, acidic agents and salts. The percentage of yield casein of the sample is 74.30%. References Ahluwalia, V. and Dhingra, S. (2005). College Practical Chemistry. [Online] Google Books. Available at: http://books.google.lk/books?id=1OgRECl_nwMCpg=PA276dq=isolation+of+caseinhl=ensa=Xei=s0rBU_jZEtOzuATSzICACgved=0CCYQ6AEwAQ#v=onepageq=isolation of caseinf=false [Accessed 12 Jul. 2014]. Alberts, B., Bray, D., hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P. (2013). Essential Cell Biology, Fourth Edition. [Online] Google Books. Available at: http://books.google.lk/books?id=Cg4WAgAAQBAJpg=PA371dq=proteins+and+hydrophilic+and+hydrophobichl=ensa=Xei=oj7BU-jdAYaUuATRyYCwDgved=0CB4Q6AEwAA#v=onepageq=proteins and hydrophilic and hydrophobicf=false [Accessed 12 Jul. 2014]. Burgess,, R. and Deutscher, M. (2009). Guide to Protein Purification. [Online] Google Books. Available at: http://books.google.lk/books?id=f6Lp4yna4hoCpg=PA118dq=protein+precipitationhl=ensa=Xei=W-jAU4icLtChugSK0IDoDgredir_esc=y#v=onepageq=protein precipitationf=false [Accessed 12 Jul. 2014]. Chaplin, M. (2014). Home | London South Bank University. [Online] Www1.lsbu.ac.uk. Available at: http://www1.lsbu.ac.uk/ [Accessed 13 Jul. 2014]. Fox, P. and McSweeney, P. (2003). Advanced Dairy Chemistry: Volume 1: Proteins, Parts AB. [Online] Google Books. Available at: http://books.google.lk/books?id=RMNkAc5PkVECpg=PA5dq=isoelectric+precipitation+of+proteinhl=ensa=Xei=MmPBU4yaIs-SuATO_IKIBgved=0CCsQ6AEwAw#v=onepageq=isoelectric precipitation of proteinf=false [Accessed 12 Jul. 2014]. Hatti-Kaul, R. and Mattiasson, B. (2003). Isolation and Purification of Proteins. [Online] Google Books. Available at: http://books.google.lk/books?id=CdHn45QCU_8Cpg=PA226dq=protein+precipitationhl=ensa=Xei=W-jAU4icLtChugSK0IDoDgredir_esc=y#v=onepageq=protein precipitationf=false [Accessed 12 Jul. 2014]. Miller, G., Jarvis, J. and McBean, L. (2006). Handbook of Dairy Foods and Nutrition, Third Edition. [Online] Google Books. Available at: http://books.google.lk/books?id=5tleQ0aLJvoCpg=PA1dq=components+of++milkhl=ensa=Xei=RQzCU8_-EI-_uAS2kYCQBwved=0CD0Q6AEwBw#v=onepageq=components of milkf=false [Accessed 13 Jul. 2014]. Rashmi, J. (2002). A Textbook of Practical Biochemistry. [Online] Google Books. Available at: http://books.google.lk/books?id=wBfAshZ3ZaUCprintsec=frontcoverdq=bio+chemistry+lab+practicalshl=ensa=Xei=XGrBU6i9ENWRuASr9oL4Bwved=0CDMQ6AEwAQ#v=onepageq=bio chemistry lab practicalsf=false [Accessed 12 Jul. 2014]. Rosenburg, (2006). Protein Analysis and Purification. [Online] Google Books. Available at: http://books.google.lk/books?id=gi-UgCF8G6ECpg=PA143dq=protein+precipitationhl=ensa=Xei=W-jAU4icLtChugSK0IDoDgredir_esc=y#v=onepageq=protein precipitationf=false [Accessed 12 Jul. 2014]. Shankara, (2008). Practical Biochemistry 2008. [Online] Google Books. Available at: http://books.google.lk/books?id=nPphGdHO_XcCprintsec=frontcoverdq=bio+chemistry+lab+practicalshl=ensa=Xei=XGrBU6i9ENWRuASr9oL4Bwved=0CC0Q6AEwAA#v=onepageq=bio chemistry lab practicalsf=false [Accessed 12 Jul. 2014]. Spurlock, D. (2014). Deborah Spurlocks Chemistry Homepage. [Online] Homepages.ius.edu. Available at: http://homepages.ius.edu/DSPURLOC/ [Accessed 12 Jul. 2014]. Tartà ©, R. (2009). Ingredients in Meat Products. [Online] Google Books. Available at: http://books.google.lk/books?id=C-wrQaaXxj0Cpg=PA132dq=principle+of+milk+proteinhl=ensa=Xei=lgjCU_DhDZGTuASf_oGoAgved=0CCcQ6AEwAQ#v=onepageq=principle of milk proteinf=false [Accessed 13 Jul. 2014]. Walsh, G. (2002). Proteins. [Online] Google Books. Available at: http://books.google.lk/books?id=EXTEjL2wTnYCprintsec=frontcoverdq=proteinhl=ensa=Xei=oEjBU4nXFZeTuAS62YGYDQved=0CD8Q6AEwBg#v=onepageq=proteinf=false [Accessed 12 Jul. 2014].

What Should Be the Relationship Between Teachers and Students? :: Argumentative Persuasive Essays

What Should Be the Relationship Between Teachers and Students? Classroom by definition is a place where students meet to study the same subject. A teacher, who took a leadership role in leading discussions and getting students to be involved in learning the subject taught, controlled this environment at one time. Now a days, the role of a teacher in a classroom environment has been reversed. Students are allowed to take the leadership role in creating and leading discussions. Just as roles of the teacher and a student have changed in a classroom over the years, so have the relationships between a student and a teacher. Many years ago, as we have read and heard, student teacher relationships have been very formal, distant, and the teacher's judgement was accepted. With almost 21 years of experience as a student, I have come to learn the relationship between student and teacher can vary depending on many characteristics. Since everyone has a different background and a different character, it is almost difficult to say how a teacher should relate t o a student or vice versa. I do believe that student teacher relationship should be professional, yet not so professional that students fear to approach. Teacher, student relationship should be formal, yet inviting. Teacher student relationship should definitely not be judgmental, and it should allow the teacher on her or his part to take time to get to know the student. At one time, about six years ago I took an economics course, which was a lower division course, it was apparently taught by one of the professors who had a very reputable standing in the department of economics. I would never forget how this professor always came to work looking very professional, wearing attire that did not seem to suit the environment. He was overly professional looking compared to other professors on campus, so it made him stand out. This look apparently had students talking about how unapproachable this professor was. No one dared to approach and ask him a question because it would only make anyone appear foolish if they did. His attitude at the beginning of the year had also thrown off many students. The fact that he would say to a student just coming into the classroom wearing his hat backwards, "Those who wear their hats backwards, their brain is backwards!" I personally did not get to know this professor because it was obvious that his intent was to just lect ure and leave.

Hamlet: What If

He would then later not have seen Aphelia being carried off into her grave. Here Hamlet fought with Alerts about how many crocodiles that they would eat to prove their love to Aphelia. Alerts would not have been back if Aphelia and Polonium had not died. This anger during this funeral lead This duel is an integral part of the play because of the very many events that occur in correlation to one another. Prior to the duel, Claudia and Alerts set up the several ways and backups for Hamlet to be killed. First they poisoned and sharpened the epee that Alerts would use.If Alerts did not strike Hamlet, they would use a poisoned chalice that Hamlet could drink from between rounds. During the duel, Hamlet wins the first two rounds (which does not matter), refusing the chalice twice. If he did not refuse, then Gertrude would have never drank from it, killing herself. A controversial topic is why she drank from it. Did she not know better? Was she suicidal? Did she do it to save Hamlet? Hamlet knew that Claudia was out to get him after his mother died to the poisoned chalice that was offered to him.In the third round, in the confusion of rage, he is poked by Alerts' poisoned epee. â€Å"It is here, Hamlet. Hamlet, thou art slain; No medicine in the world can do thee good. In thee there is not half an hour of life. The treacherous instrument is in thy hand, Unabated and envenomed. The foul practice Hath turned itself on me. Lo, here I lie, Never to rise again. Thy mother's poison's. I can no more. The King, the King's to blame. â€Å"Knowing the imminence of death, Hamlet then killed King Claudia of Denmark using he poisoned epee out of revenge for both of his parent's.Alerts dies also from the poison-point. Hamlet is the last to die from the poison, in Hortatory arms, naming Fortifiers the new king of Denmark. This is the only way the duel scene could have played out, the only variables were Gertrude drinking the poison (and her knowledge of it) and Hamlet getting poke d by Alerts' poisoned point. If Gertrude DID know about the conspiracy to kill Hamlet, she could have been possible for to have thrown herself in front of Alerts' point. Either way, she would not have gotten out alive.If here was no way for her to live, then Hamlet would have always killed Claudia. If Hamlet did not get poisoned, then he would have ruled Denmark. However, the â€Å"hero† living at the end of a tragedy is not characteristic of Shakespearean plays. Hamlet would have probably killed himself at the end, anyways, with his mother, lover, and still yet his father, dead. Shakespeare was well aware of how multiple outcomes could play out and probably made a hundred drafts of his play, trying to make it the most attention- grabbing possible. He, as proven, had most definitely succeeded in doing so.

Atlantic Telegraph Cable Timeline

Atlantic Telegraph Cable Timeline The first telegraph cable to cross the Atlantic Ocean failed after working for a few weeks in 1858. The businessman behind the audacious project, Cyrus Field, was determined to make another attempt, but the Civil War, and numerous financial problems, interceded. Another failed attempt was made in the summer of 1865. And finally, in 1866, a fully functional cable was placed that connected Europe to North America. The two continents have been in constant communication since. The cable stretching thousands of miles under the waves changed the world profoundly, as news no longer took weeks to cross the ocean. The nearly instant movement of news was a huge leap forward for business, and it changed the way Americans and Europeans viewed the news. The following timeline details  major events in the long struggle to transmit telegraphic messages between continents. 1842: During the experimental phase of the telegraph, Samuel Morse placed an underwater cable in New York Harbor and succeeded in sending messages across it. A few years later, Ezra Cornell placed a telegraph cable across the Hudson River from New York City to New Jersey. 1851: A telegraph cable was laid under the English Channel, connecting England and France. January 1854: A British entrepreneur, Frederic Gisborne, who had run into financial problems while trying to place an undersea telegraph cable from Newfoundland to Nova Scotia, happened to meet Cyrus Field, a wealthy businessman and investor in New York City. Gisbornes original idea was to transmit information faster than ever between North America and Europe by employing ships and telegraph cables. The town of St. Johns, on the eastern tip of the island of Newfoundland, is the closest point to Europe in North America. Gisborne envisioned fast boats delivering news from Europe to St. Johns, and the information quickly being relayed, via his underwater cable, from the island to the Canadian mainland and then onward to New York City. While considering whether to invest in Gisbornes Canadian cable, Field looked closely at a globe in his study. He was struck with a far more ambitious thought: a cable should continue eastward from St. Johns, across the Atlantic Ocean, to a peninsula jutting into the ocean from the west coast of Ireland. As   connections were already in place between Ireland and England, news from London could then be relayed to New York City very quickly. May 6, 1854: Cyrus Field, with his neighbor Peter Cooper, a wealthy New York businessman, and other investors,  formed a company to create a telegraphic link between North America and Europe. The Canadian Link 1856: After overcoming many obstacles, a working telegraph line finally reached from St. Johns, on the edge of the Atlantic, to the Canadian mainland. Messages from St. Johns, on the edge of North America, could be relayed to New York City. Summer 1856: An ocean expedition took soundings and determined that a plateau on the ocean floor would provide a suitable surface on which to place a telegraph cable. Cyrus Field, visiting England, organized the Atlantic Telegraph Company and was able to interest British investors to join the American businessmen backing the effort to lay the cable. December 1856: Back in America, Field visited Washington, D.C., and convinced the U.S. government to assist in the laying of the cable. Senator William Seward of New York introduced a bill to provide funding for the cable. It narrowly passed through Congress and was signed into law by President Franklin Pierce on March 3, 1857, on Pierces last day in office. The 1857 Expedition: A Fast Failure Spring 1857: The U.S. Navys largest steam-powered ship, U.S.S. Niagara sailed to England and rendezvoused with a British ship, H.M.S. Agamemnon. Each ship took on 1,300 miles of coiled cable, and a plan was devised for them to lay the cable across the bottom of the sea. The ships would sail together westward from Valentia, on the west coast of Ireland, with the Niagara dropping its length of cable as it sailed. At mid-ocean, the cable dropped from the Niagara would be spliced to to the cable carried on the Agamemnon, which would then play out its cable all the way to Canada. August 6, 1857: The ships left Ireland and began dropping the cable into the ocean. August 10, 1857: The cable aboard the Niagara, which had been transmitting messages back and forth to Ireland as a test, suddenly stopped working. While engineers tried to determine the cause of the problem, a malfunction with the cable-laying machinery on the Niagara snapped the cable. The ships had to return to Ireland, having lost 300 miles of cable at sea. It was decided to try again the following year. The First 1858 Expedition: ANew Plan Met New Problems March 9, 1858: The Niagara sailed from New York to England, where it again stowed cable on board and met up with the Agamemnon. A new plan was for the ships to go to a point mid-ocean, splice together the portions of cable they each carried, and then sail apart as they lowered cable down to the ocean floor. June 10, 1858: The two cable-carrying ships, and a small fleet of escorts, sailed out from England. They encounter ferocious storms, which caused very difficult sailing for ships carrying the enormous weight of cable, but all survived intact. June 26, 1858: The cables on Niagara and Agamemnon were spliced together, and the operation of placing the cable began. Problems were encountered almost immediately. June 29, 1858: After three days of continuous difficulties, a break in the cable made the expedition halt and head back to England. The Second 1858 Expedition: Success Followed By Failure July 17, 1858: The ships left Cork, Ireland, to make another attempt, utilizing essentially the same plan.   July 29, 1858: At mid-ocean, the cables were spliced and Niagara and Agamemnon began steaming in opposite directions, dropping the cable between them. The two ships were able to communicate back and forth via the cable, which served as a test that all was functioning well. August 2, 1858: The Agamemnon reached Valentia harbor on the west coast of Ireland and the cable was brought ashore. August 5, 1858: The Niagara reached St. Johns, Newfoundland, and the cable was connected to the land station. A message was telegraphed to newspapers in New York alerting them of the news. The message stated that the cable crossing the ocean was 1,950 statue miles long. Celebrations broke out in New York City, Boston, and other American cities. A New York Times headline declared the new cable The Great Event of The Age. A congratulatory message was sent across the cable from Queen Victoria to President James Buchanan. When the message was relayed to Washington, American officials  at first believed the message from the British monarch to be a hoax. September 1, 1858: The cable, which had been operating for four weeks, began failing. A problem with the electrical mechanism that powered the cable proved fatal, and the cable stopped working entirely. Many in the public believed it had all been a hoax. The 1865 Expedition: New Technology, New Problems Continued attempts to lay a working cable were suspended due to a lack of funds. And the outbreak of the Civil War made the entire project impractical. The telegraph played an important role in the war, and President Lincoln used the telegraph extensively to communicate with commanders. But extending cables to another continent was far from a wartime priority. As the war was coming to an end, and Cyrus Field was able to get financial problems under control, preparations began for another expedition, this time using one enormous ship, the Great Eastern. The ship, which had been designed and built by the great Victorian engineer Isambard Brunel, had become unprofitable to operate. But its vast size made it perfect for storing and laying telegraph cable. The cable to be laid in 1865 was made with higher specifications than the 1857-58 cable. And the process of putting the cable aboard ship was greatly improved, as it was suspected that rough handling on the ships had weakened the earlier cable. The painstaking work of spooling the cable on the Great Eastern was a source of fascination for the public, and illustrations of it appeared in popular periodicals. July 15, 1865: The Great Eastern sailed from England on its mission to place the new cable. July 23, 1865: After one end of the cable was fashioned to a land station on the west coast of Ireland, the Great Eastern began to sail westward while dropping the cable. August 2, 1865: A problem with the cable necessitated repairs, and the cable broke and was lost on the sea floor. Several attempts to retrieve the cable with a grappling hook failed. August 11, 1865: Frustrated by all attempts to raise the sunken and severed cable, the Great Eastern began to steam back to England. Attempts to place the cable that year were suspended. The Successful 1866 Expedition: June 30, 1866:  The Great Eastern steamed from England with new cable aboard. July 13, 1866:  Defying superstition, on a Friday the 13th the fifth attempt since 1857 to lay the cable began. And this time the attempt to connect the continents encountered very few problems. July 18, 1866: In the only serious problem encountered on the expedition, a tangle in the cable had to be sorted out. The process took about two hours and was successful. July 27, 1866: The Great Eastern reached the shore of Canada, and the cable was brought ashore. July 28, 1866: The cable was proven successful and congratulatory messages began to travel across it. This time the connection between Europe and North America remained steady, and the two continents have been in contact, via undersea cables, to the present day. After successfully laying the 1866 cable, the expedition then located, and repaired, the cable lost in 1865. The two working cables began to change the world, and over the following decades more cables crossed the Atlantic as well as other vast bodies of water. After a decade of frustration the era of instant communication had arrived.

Texas Holdem Rules Everything You Need to Win

Texas Holdem Rules Everything You Need to Win SAT / ACT Prep Online Guides and Tips Imagine this scenario: your friends invited you to a poker night. There's going to be food, booze, and poker. You want to join in on the fun, but you have no idea how to play poker. Well don't worry about missing out on beer and bonding: I'm going to teach you how to play Texas Holdem, so you too can partake in the poker festivities. Texas Holdem (aka Texas Hold 'Em) is the poker game played most often at casinos and home poker games. Becoming a great Holdem player generally takes years of practice and studying, but learning how to play doesn’t take long at all. In this article, I’ll walk you through the Texas Holdem rules. After studying this article, you’ll be able to play online, at a casino, or with your friends. Hooray! Remember, just knowing the rules won’t make you an outstanding player, but you’ll be able to play, and you can get better through practice. How to Win Texas Holdem The goal in poker is to win as many poker chips as you can, one hand at a time. Typically, different chips will represent different denominations of money. You can win chips by having the best 5-card hand or remaining in a hand and having all the other players fold, meaning they give up instead of risking additional chips. The 10 Possible Poker Hands In Holdem, each player is dealt 2 cards (â€Å"hole cards†). Then, up to 5 community cards will be flipped in the center of the table. I’ll thoroughly explain the concept of community cards later, but Texas Holdem rules dictate that your best 5-card hand out of the 7 total cards is your final hand. These are how all the different poker hands rank. The Royal Flush is the best hand and the worst is â€Å"high card.† #1: Royal Flush: A, K, Q, J, 10 all the same suit (all clubs, all diamonds, all spades, or all hearts) #2: Straight Flush: Five cards in a sequence (example: 5, 6, 7, 8, 9), all the same suit #3: Four of a Kind: Four cards of the same rank (example: four kings or four 8’s) #4: Full House: Three of a kind and a pair (example: three jacks and two 7’s) #5: Flush: Five cards of the same suit (example: five clubs or five hearts) #6: Straight: Five cards in a sequence but not of the same suit (example: 7, 8, 9, 10, J) #7: Three of a Kind/A Set: Three cards of the same rank (example: three 5’s or three kings) #8: Two Pair: Two different pairs (example: two 6’s and two 9’s) #9: Pair: Two cards of the same rank (example: two queens or two 10’s) #10: High Card: If you haven’t made any of the above hands, then your highest card determines the strength of your hand. (Example: if your highest card is an Ace, you have Ace high, and you would beat another hand in which the highest card was a King and none of the other hands were made.) If multiple players have hands that are ranked the same, the best hand is determined by the highest card within the hand. For example, if each player has a pair, then the highest pair wins. If somebody has a pair of 7’s and somebody has a pair of 5’s, the player with the pair of 7’s has a better hand. If two players have a pair of 7’s, then the player with the highest other card wins. If you're comparing two straights or flushes, then the player with the highest card within the straight or flush has the better hand. If you're comparing two full houses, the player with the highest card in the three of a kind has the better hand. In most hands of Holdem, the winning player will only have a pair, but if you play long enough, you'll see every type of hand. A straight! Texas Holdem Setup Holdem can be played with 2-10 players at a single table. Players sit around the table. Before beginning playing, each player purchases his chips. In a home game or tournament, everyone typically starts with the same number of chips. Usually, each player will put in the same amount of money and get the same number of chips. For example, everyone may put in $40 and get $2,000 in chips. At a home game, you can decide if the winner (person who ends up with all the chips or the most chips) takes all, or if you’ll end up dividing the money among the top 2-3 finishers. Each tournament has its own guidelines. At a regular casino game, the number of chips you get is based on your buy-in (the amount of money you give the dealer to receive your chips). If you spend more than the minimum buy-in, you’ll get more chips. If you buy in with $100, you’ll get $100 worth of chips. If you buy in with $200, you’ll get $200 worth of chips. At most casinos, there will be a minimum and maximum buy-in. Generally, the minimum buy-in will be 50 or 100 times the minimum bet. The Dealer Button At a home game, the dealer button is placed in front of the person who will play the role of the dealer for that hand and bet last after the initial round of betting. After each hand, the dealer button moves to the left. At a casino, there will be a casino employee who will deal each hand, and the dealer button is solely used to determine who will bet last. Again, after each hand, the dealer button moves to the left. Here’s what the dealer button looks like: John Wardell/Flickr If you’re playing a home game and don’t have an official dealer button, you can use any object to function as the dealer button. Just make sure not to use one of the same color chips that you’re using to place bets, so you don’t confuse the dealer button with a bet. The Blinds Blinds are the initial bets at the table. At the beginning of each hand, two players will post bets before any cards are dealt. These bets are referred to as the small blind and the big blind. The player to the left of the dealer button posts the small blind and the player to the left of the small blind posts the big blind. To post your blind, just place your blind in front of you on the table. The big blind is the minimum bet after cards are dealt, and the small blind is half of that. In casinos, at the tables with the lowest minimums, small blinds are usually $1 and big blinds are $2. These games are referred to as â€Å"$1-$2 holdem.† Most casinos will also offer â€Å"2-4 holdem† and â€Å"3-6 holdem.† The first number is the small blind and the second is the big blind. The purpose of the blinds is to stimulate betting. The blinds guarantee that there will be chips to win in each hand. Also, extremely conservative players are forced to make bets and can't just play for hours without risking any chips. Betting Rounds Texas Holdem involves four different rounds of betting. In each round, you normally have 3 options: you can fold, raise, or call. If you raise, you increase the bet by at least the big blind. In no limit poker, the most common type for casinos and home games, you can bet up to all of your chips. If you bet all of your chips, that’s called going all-in. In limit poker, you can only raise by a predetermined maximum amount (normally twice the big blind), and there’s only a certain number of raises allowed per betting round (usually 4-5 bets per round). If you fold, you’re giving up your hand instead of betting additional chips. When you fold, you put your cards face down in the center of the table. Never show your cards to anybody else, even when you fold. If you call, you’re matching the last bet. If somebody bets $2 and the next person raises to $8, you have to bet $8 to call. Additionally, if nobody has bet in a round, you can â€Å"check.† If you check (just say â€Å"check† or knock on the table), you’re keeping your hand and going to the next person without placing a bet. Typically, people check if they have a weak hand or they want the other players to think they have a weak hand (they’re trying to get other players to bet). You can also check if you’re the big blind and nobody has raised the big blind by the time betting action reaches you. Betting Round 1: The Pre-Flop The pre-flop betting round happens after each player receives his 2 cards and before any community cards are turned. Cards are dealt to the left (clockwise), one at a time, starting with the small blind. Betting action starts to the left of the big blind. That player has the option of calling the big blind, raising, or folding. After each person has had a turn and all remaining players have bet the same amount, the betting round is over. If somebody raises, the next person has to call the raise, raise again, or fold. If nobody raises the big blind, the big blind, who is the last person to act, can check and the betting round will be completed. But the big blind still has the option to raise. If he raises, then the remaining players will have to call, raise again, or fold. Players bet by placing their chips in front of them. At the end of the round, all the chips that have been bet go into the center of table and are referred to as â€Å"the pot.† At any point during any of the betting rounds, if all players fold, then the last remaining player wins the pot and the hand is completed. You start a new hand instead of continuing with one player. Betting Round 2: The Flop Three community cards are flipped. Every time community cards are flipped, the dealer should use a â€Å"burn card.† The burn card is the very top card on the deck. The burn card will be placed face down, and then the next three cards will be placed face up in the center of the table and will be the flop. The flop is the first three community cards. After the flop is dealt, there’s another betting round. Action will start with the first remaining player to the left of the dealer button. The same betting rules apply: players can bet, fold, or call. If nobody has placed a bet, a player can check. Again, once each player has had a turn and everyone has bet the same amount, the betting round is completed. If you have the final 7, this is a very lucky flop! (auddess/Flickr) Betting Round 3: The Turn The dealer burns a card and then flips the top card next to the flop. The turn is the fourth community card. Then, there’s another round of betting. All the same betting rules apply. Again, action starts with the first remaining player to the left of the dealer button. Betting Round 4: The River After burning a card, the dealer flips the top card next to the turn. The river is the last of the five community cards. Then, there’s one final betting round. All the same rules from the previous betting rounds are used. If there’s more than one hand remaining at the end of the round, there’s a showdown. The Showdown In a showdown, the remaining players show their hole cards, and the best hand wins the pot. The best hand is determined by comparing each player’s best 5-card hand from their 2 hole cards and the 5 community cards. If their hands are ranked the same, the player with the highest card within the hand wins. If the players have identical 5-card hands, then the players will split the pot. Also, if you don’t want to show your cards, you can â€Å"muck† your cards (place them face down in the center of the table) and automatically lose. You can do this if another player shows a better hand and you don’t want the other players to know how bad or good your hand was. In the showdown, you put all your cards on the table. Literally. (Big Daddy Brent/Flickr) How to Play Texas Holdem: Summary In Holdem, every player gets two cards, called â€Å"hole cards,† and the rest of the cards are â€Å"community cards,† which means they’re used by each player to produce the best possible five card hand. After the blinds are posted, each player is dealt two cards face down. Cards are dealt one at a time, clockwise, starting with the small blind. After everyone looks at their hole cards (never show your cards to anyone else), there’s a round of betting followed by â€Å"the flop.† For the flop, three cards are flipped from the top of the deck in the center of the table. After the flop, there’s another round of betting. Then, there’s â€Å"the turn.† For â€Å"the turn,† one additional card is flipped and placed next to the last card of the flop. Then, there’s another round of betting. Finally, there’s â€Å"the river.† For the river, one last community card is flipped and placed next to the turn. The flop, turn, and river are the five community cards. Players use their hole cards and the community cards to determine their best 5-card hand. After the river, there’s one final round of betting. If there’s more than one player remaining after the final round of betting, players show their hole cards, and the best 5-card hand wins the the pot. If all players fold before the end of the last betting round, the last remaining player wins the pot. Texas Holdem Rules Video Tutorials Here are a few video tutorials so you can see the Texas Holdem rules we just discussed in action. This first video quickly goes through the Holdem rules in under four minutes: This video is slightly longer and provides more explanation of how to play Texas Holdem: Finally, this video takes you through a complete hand of Holdem, discusses some basic strategy, and shows how you can lose with a seemingly great hand: 4 Very Basic Texas Holdem Strategies Now that you've got the basic Texas Holdem rules down, it's time to start thinking about strategy. As you become a better player, you can vary your play and develop an individual style, depending on what works best for you. However, following these strategies as a beginning player will normally give you your best odds of winning or minimizing your losses. Remember that these are just general guidelines for beginners to simplify the game. Play Tight (Especially Pre-Flop) You should only place a bet pre-flop if you have a good starting hand. Generally, good starting hands are pairs, at least one Ace, or two face cards. If you’re in the small blind, if there isn’t a raise, it’s typically in your best interest to call the big blind and see the flop. After the flop, don’t bet or call unless you have at least a pair. One notable exception is if you have at least 4 of the same suit or 4 in a sequence. Then, you have a decent possibility to make a flush or a straight. But if there’s a big bet or raise ahead of you, you may not want to call just to chase a flush or a straight. If it’s a small bet, it’s probably worth it to call. Play Aggressively When You Have a Good Hand This means that if you have a good hand, you should raise or bet. If you have a good hand pre-flop, raise 2-3 times the big blind. If you have a good hand post-flop, raise at least half of the pot. A good hand post-flop is at least top pair, meaning that one of your hole cards pairs with the highest ranked community card. If you have a good hand post-flop, don’t just check or call. You should bet or raise. You can try to get fancy and check raise, meaning that you check, and then after a player bets, you raise his bet. But normally only more advanced players use this move. If You’re the Dealer, Use Your Position to Your Advantage If you’re on the button (the dealer) or close to it, you can decide what to do based on the other players’ decisions. If everyone in front of you checks, it’s possible that nobody has a good hand and you can try to take the pot by betting. The assumption being that if you bet, everyone else will fold. If multiple people in front of you bet or raise, you can assume that somebody has a strong hand, and you should only call or raise if you have a very strong hand. Don’t Let the Outcome of Previous Hands Determine How You Play Your Current Hand In poker, even if you make the right play, it’s possible for bad things to happen. For example, if you fold a 5-7 pre-flop and three 7’s come out on the flop, that doesn’t mean that you made the wrong decision, especially if there was a raise before you folded. The odds of three 7’s coming out on the flop were incredibly low. Similarly, if you bet a good hand on the turn, then a weaker hand calls and ends up beating you by getting a lucky card on the river, you didn't make the wrong decision when you bet after the turn. It's important not to let unlucky or lucky outcomes in previous hands influence your decisions. Focus on making the right play in that situation, and you'll give yourself the best chance to win.

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